Tissue-Stat ( Tissue Adherent for Mounting Formalin-Fixed and Frozen Tissue Sections.)
While performing immunocytochemical procedures, the proper attachment of the tissue sections to the glass slides is an essential requirement. Poorly mounted specimens will tend to yield questionable and inferior results. Various animal proteins such as gelatin, ovalbumin and partially digested casein have been used to promote tissue adherence to glass surfaces. However, some of these proteins may interfere in the immunocytochemical assays or may produce undesirable non-specific backgound staining. A polymeric basic amino acid, polylysine, has also been used for this purpose but the high price of this substance has hampered its widespread use.
Tissue-Stat is a positively charged silicone derivative. This product is used to treat the surface of slides destined for immunocytochemical staining. The proper adherence of the tissue sections to the glass slides is an essential requirement for successful staining. Poorly mounted specimens will tend to yield questionable and inferior stainings. Various animal proteins such as gelatin, ovalbumin and partially digested casein have been used to promote tissue adherence to glass surfaces. However, some of these proteins may interfere in the immunocytochemical assays or produce undesirable nonspecific background staining. Glass slides treated with Tissue-Stat can be stored for several months at room temperature in a ready-to-use form until needed.
Refrigerate at 4°C. Do not freeze.
Preparation of Tissue-Stat Working Solution:
Perform Steps 1-5 in Fume Hood
1.Transfer 250 mL of Acetone (ACS Reagent Grade) into a teflon or glass capped glass bottle.
2.Add the entire contents of the concentrated Tissue-Stat vial to the bottle. Cap the bottle and mix the solution by gently swirling the bottle. Store this solution at room temperature in the fume hood. This solution has a shelf-life of 3-6 months if kept sealed and inside a fume hood.
OR ADD ONE DROP OF TISSUE STAT TO 1 ML OF ACETONE OR 5 DROPS TO 5 ML OF ACETONE.
3. Transfer Tissue-Stat/acetone mixture into a vertical solvent-resistant jar.
4. Dip slide in this solution for aproximatly 30 seconds.
5. Remove slides, touch the side of the slide on paper bllotting towel to remove excess solution. Bake in 70°C-80°C oven for 5-10 minutes. These slides are ready to be used, and can be stored at room temperature for further use.
Materials needed but not supplied: - Distilled or deionized water.
- Acetone (ACS Reagent Grade)
- Vertical solvent-resistant staining jars.
- Solvent-resistant vertical slide holders to fit into above staining jars.
- Oven for 70-80°C (Optional).