The ELISpot reader is different from conventional ELISA readers on reading methods and analysis softwares. In ELISpot readers normally it contains built-in microscope to effectively collect ELISpot signals.
Otherwise there are special ELISpot readers that can read fuorescence signals or fit for 24-well, 12-well, even 6-well plates. By present most of there ELISpot readers are specifically pruduced by certain manufacturers.
Corresponding ELISpot software automatically count posotive signals on the membrane. Furthermore, difference among spots can be campared and shown in software windows.