Blood Group Antigen A - b480 - Prediluted

Monoclonal Mouse Anti-Human Blood Group A

This antibody is prediluted for Immunohistochemistry use.

Pure endocervical ovarian cyst mucin

Recommended Positive Control:
Human Tissues

Mouse monoclonal antibody prediluted in Primary Antibody Diluting Buffer pH 7.6.

Aliquoting Instructions:
Not applicable

Staining Procedure:
The prediluted antibody can be used on frozen cryostat sections as well as formalin-fixed paraffin-embedded tissue sections. For paraffin-embedded tissue sections, we recommend an incubation time and temperature of 30 minutes at 37°C for this antibody, when used in conjunction with Biømeda’s immunoperoxidase staining kit. *Prolonged fixation in buffered formalin can destroy the epitope.

This antibody reacts with blood group substance A, subtype A1 and A2, on the cell membranes of epithelium, endothelium and red blood cells.

This antibody is compatible with the following Biømeda staining kits: AutoProbe III Wide Spectrum Universal Staining Kits (Cat. No. 08-803, 08-804X), HistoScan Monoclonal Detector (Cat. No. 06-601, 06-601A and 06-601F), BiøStain Super ABC Mouse/Rat Kit (Cat. No. 11-002 and 11-002A), UltraProbe High Sensitivity Staining Kits (Cat. No. 09-900, 09-901, 09-901X).

Refrigerate at 4°C. Do not freeze.

1. Chapman et al., J Histochem Cytochem, 31: 557, 1983.
2. Biochem. J. 254:185-193, 1988.
3. Glycoconjugate 3:187, 202 ().