Blood Group Antigen A - b480 - Prediluted
Description:
Monoclonal Mouse Anti-Human Blood Group A
Application:
This antibody is prediluted for Immunohistochemistry use.
Immunogen:
Pure endocervical ovarian cyst mucin
Recommended Positive Control:
Human Tissues
Presentation:
Mouse monoclonal antibody prediluted in Primary Antibody Diluting Buffer pH 7.6.
Aliquoting Instructions:
Not applicable
Staining Procedure:
The prediluted antibody can be used on frozen cryostat sections as well as formalin-fixed paraffin-embedded tissue sections. For paraffin-embedded tissue sections, we recommend an incubation time and temperature of 30 minutes at 37°C for this antibody, when used in conjunction with Biømeda’s immunoperoxidase staining kit. *Prolonged fixation in buffered formalin can destroy the epitope.
Specificity:
This antibody reacts with blood group substance A, subtype A1 and A2, on the cell membranes of epithelium, endothelium and red blood cells.
Compatibility:
This antibody is compatible with the following Biømeda staining kits: AutoProbe III Wide Spectrum Universal Staining Kits (Cat. No. 08-803, 08-804X), HistoScan Monoclonal Detector (Cat. No. 06-601, 06-601A and 06-601F), BiøStain Super ABC Mouse/Rat Kit (Cat. No. 11-002 and 11-002A), UltraProbe High Sensitivity Staining Kits (Cat. No. 09-900, 09-901, 09-901X).
Storage:
Refrigerate at 4°C. Do not freeze.
References:
1. Chapman et al., J Histochem Cytochem, 31: 557, 1983.
2. Biochem. J. 254:185-193, 1988.
3. Glycoconjugate 3:187, 202 ().