• Wide range of detection (e.g., cytokines and granzyme B)
• High throughput, high content analysis
• Lower limit of detection
• Applicability to frozen/thawed biological samples
• Affordable price
When compared to other immunoassay techniques such as flow cytometry, ELISpot is considered to be preferable for its higher sensitivity. It has been supposed that a typical ELISpot assay is 100–200-fold more sensitive than conventional ELISA in the detection of the secreted cytokines. For the sake of being cheaper and time-saving, the ELISpot could be improved to multi-color FluoroSpot.
ELISpot (Advantage) Reference:
 Karlsson AC, et al., Comparison of the ELISPOT and cytokine flow cytometry assays for the enumeration of antigen-specific T cells, J Immunol Methods. 2003, 283(1-2):141-153;
 Ahlborg N & Axelsson B, Dual- and triple-color fluorospot, Methods Mol Biol. 2012, 792:77-85.