Most of the fixatives immobilize the tissue antigens by forming cross-linkages. Sometimes extensive cross-linking of proteins in high concentrations can also lead to the masking or destruction of antigens, which leads to poor staining or no staining at all. This loss of staining can be restored to certain extent by pretreatment of tissue sections with proteolytic enzymes. The unmasking of tissue sections is an important requirement for the optimal staining of antigens like immunoglobulins, Keratins, Carcinoembyonic Antigen, Factor VIII-related Antigen, testosterone and to a lesser degree S-100 Protein.
Do not use this Pepsin Reagent when detecting Cytokeratins using the AE3 monoclonal antibody Clone; instead use Auto/Zyme.
Pepsin Reagent is a stabilized enzyme designed for the uncovering of tissue antigens fixed with formalin.
Pepsin Reagent is a stabilized enzyme designed for the uncovering of tissue antigens fixed with formalin. This reagent was developed by David J. Brigati, M.D. Pepsin Reagent should not be used when cytokeratin AE1/AE3 is employed. Use Auto/Zyme instead.
Refrigerate at 4°C. Do not freeze.
Use of Pepsin Reagent: The Pepsin reagent is supplied in a ready-to-use form requiring no further preparation:
1. Apply this solution to tissue sections following their initial hydration step. Incubate for 10 minutes at 37°C or 45°C.*
2. Wash tissue sections well with 1X Automation Buffer. **
* This incubation time may vary depending on the degree of fixation of the tissue sections.
** Biomeda's 10X Automation Buffer (Cat. No. M30).
Do not use beyond the expiration dated stated on the label.
For Research Use Only.