Microphthalmia (Transcription Factor) (MiTF)
Description:
Mi is a basic helix-loop-helix-leucin zipper (b-HLH-ZIP) transcription factor implicated in pigmentation, mast cells, and bone development. The mutation of Mi causes Waardenburg Syndrome type II in humans.
Application:
- Gel Supershift (Order Ab at 1mg/ml)
- Western Blotting - Not verified
- Immunohistology (Formalin/paraffin)(Use Ab at 4-8 µg/ml for 30 min at RT)
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
-THE OPTIMAL DILUTION SHOULD BE DETERMINED BY THE INDIVIDUAL LAB.
Immunogen:
N-terminal fragment of human Mi protein
Cellular Localization:
Nuclear
Species Reactivity:
Human, Dog. Does not react with Mouse, and Rat. Others not tested.
Recommended Positive Control:
501 Mel Cells (1), Melanoma
Epitope:
N-terminal
Presentation:
200ug/ml of antibody purified from ascites fluid by Protein G chromatography. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide. Also available without BSA and azide at 1mg/ml.
Storage:
Refrigerate at 4°C. Do not freeze.
Notes:
This ab. does not cross-react with other b-HLH-ZIP factors by DNA mobility shift assay. This ab. reacts with both melanocytic and nonmelanocytic isoforms of Mi. It is especially useful for research on murine Mi protein.
Epitope: N-terminal
References:
1. Weilbaecher KN, et al. J. Exp.Med. 1998, 187: 775-785
2. Hemesath P, et. al. Nature. 1998, 391:298-301