Antitrypsin, Alpha-1 (AAT) - Concentrated- Mouse
Description:
This antibody reacts with the 51 kD human alpha-1-antitrypsin. It does not cross-react with alpha-1-acid glycoprotein, serum albumin, or other serum proteins. This antibody is appropriate for recognizing tissues exhibiting AAT deficiency, hepatocellular carcinoma, oral contraceptive associated hepatic tumors, endodermal sinus tumors, or AAT in histiocytes.
Application:
Immunohistochemistry (Paraffin/Frozen) 1:50
Immunogen:
Human alpha-1-Antitrypsin
Cellular Localization:
Cytoplasmic
Species Reactivity:
Human. Others not tested.
Presentation:
20 mM Tris-Borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing Fetal Bovine Serum, BMC-6 Carrier Polysaccharides, Carrier Protein, and 0.05% Sodium Azide, pH 7.5.
Aliquoting Instructions:
Do not dilute the entire reconsituted solution at once. Withdraw aliquots as needed with a micropipette and keep concentrated stock at 4°C. Dilute according to the particular application being used. In general, the 0.05M Borate pH 8.0 containing 0.15M Sodium Chloride, 0.05% Sodium Azide, is a good dilutent to use with most antibodies. GENERAL INFO: When diluting for Immunohistochemistry, ELISA, or Western Blot, make the dilution in Primary Antibody Diluting Buffer (Cat. No. M35). Avoid diluting the entire contents of the vial at once since the diluted solution may have reduced stability.
Staining Procedure:
Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50 with AutoProbe III (Cat. No. 08-803). The optimal conditions should be determined by the individual laboratory.
Compatibility:
This antibody is compatible with the following biømeda staining kits: AutoProbe III Wide Spectrum Universal Staining kits (Cat. No. 08-803, 08-804X), HistoScan Monoclonal Detector (Cat. No. 06-601, 06-601A and 06-601F.). BiøStain Super ABC Mouse/Rat kits (Cat. No. 11-002 and 11-002A), UltraProbe High Sensitivity Staining kits (Cat. 09-900, 09-901, 09-901X), Lab/Probe Mouse/Rabbit kits (Cat. No. 10-101 and 10-102) andAutoProbe II Universal kits (Cat. No. 08-801 and 08-802X).