Nuclear Fast Red Counterstain
Description:
Nuclear Fast Red Counterstain
Presentation:
Biømeda’s Nuclear Fast Red is a light red nuclear counterstain designed for counterstaining immunohistochemistry chromogens like BCIP/NBT (Cat. No. S04), BCIP/INT (Cat. No. S03), DAB Cobalt (Cat. No. S15). This is an ideal counterstain to be used with the chromogens that cannot be counterstained with hematoxylin because of the little or no color contrast. Specimens counterstained with Nuclear Fast Red can be dehydrated and mounted with organic solvents-based plastic polymers such as ClariØn (Cat. No. M05, M06) or Permount.
Specificity:
This light red nuclear counterstain is designed for counterstaining BCIP/NBT and DAB Cobalt. Specimens counterstained with Nuclear Fast Red should be dehydrated and mounted with an organic mounting media such as ClariØn (See Pg. 54). This counterstain is not compatible with aqueous mounting media.
Compatibility:
Nuclear Fast Red Counterstain is NOT compatible with Crystal/Mount, Gel/Mount or other aqueous mounting media.
Storage:
Store at room temperature.
Notes:
Seven Step Procedure: 1. After the end of the chromogen incubation, rinse slides with distilled water and drain.
2. Introduce the Nuclear Fast Red.
3. Incubate the slides for 1-2 min. at room temperature.
4. Drain off the Nuclear Fast Red and wash slides several times with distilled water.
5. Dehydrate the slides by passing them through graded alcohols.
6. Bring the slides to xylene or xylene substitute clearing agent.
7. Mount with ClariØn (Cat. No. M05, M06) or other organic solvent based mounting medium.
Upon prolonged storage, Nuclear Fast Red Counterstain has a tendency to precipitate. In such an instance, it is recommended to filter the solution using a syringe filter.
Do not use beyond the expiration date stated on the label.
FOR RESEARCH USE ONLY.
This is a laboratory reagent and should only be used by trained laboratory personnel. Avoid contact with skin and clothing.