Methylene Blue Counterstain

Methylene Blue Counterstain

Biømeda’s Methylene Blue is a light blue counterstain designed for counterstaining immunohistochemistry chromogens like DAB (Cat. No. S11), DAB Enhanced (Cat. No. S14) and DAB Cobalt (Cat. No. S15). This is an alternative counterstain that can be used in place of hematoxylin. It is an excellent counterstain for use with immunogold staining. Specimens counterstained with Methylene Blue can be dehydrated and mounted with organic solvents-based plastic polymers such as ClariØn (Cat. No. M05, M06) or Permount.

This light blue counterstain is designed for counterstaining chromogens commonly used in immunohistochemistry such as DAB, DAB Enhanced and DAB Cobalt. Methylene Blue can also be used with immunogold staining. Specimens counterstained with Methylene Blue should be dehydrated and mounted with an organic mounting media such as ClariØn. This counterstain is not compatible with aqueous mounting media.

Methylene Blue Counterstain is not compatible with Crystal/Mount, Gel/Mount or other aqueous mounting media.

Store at room temperature.

Seven Step Procedure: 1.    After the end of the chromogen incubation, rinse slides with distilled water and drain.
2.    Introduce the Methylene Blue.
3.    Incubate the slides for 1-2 min. at room temperature.
4.    Drain off the Methylene Blue and wash slides several times with distilled water.
5.    Dehydrate the slides by passing them through graded alcohols.
6.    Bring the slides to xylene or xylene substitute clearing agent.
7.    Mount with ClariØn (Cat. No. M05, M06) or other organic solvent based mounting medium.

Upon prolonged storage, Methylene Blue Counterstain has a tendency to precipitate. In such an instance, it is recommended to filter the solution using a syringe filter.

Do not use beyond the expiration date stated on the label.


This a laboratory reagent and should only be used by trained laboratory personnel. Avoid contact with skin or clothing.