Adenovirus Fiber (Trimer only)
Recognizes only trimers (high molecular weight bands 180-200 kD) on Western blots of unboiled Ad2 and Ad5 fiber proteins and shows immunofluorescence with Ad5 fiber mutant H5ts142-infected cells at 320C but not at 39.50C.1.
Adenovirus capsid proteins are synthesized in the cytoplasm and transported to the nucleus for assembly into the virus particles. The three major capsid proteins (hexon, penton base, and fiber) are synthesized late in infection.
- Inhibition of Ad2 attachment (Not suitable)(2)
- Western Blotting (Ab 1 micrograms/ml for 2hrs at RT)(1)
-THE OPTIMAL DILUTION SHOULD BE DETERMINED BY THE INDIVIDUAL LAB.
UV-irradiated Ad2 virus
Recommended Positive Control:
Adenovirus infected cells and tissues
200ug/ml antibody purified from the ascites fluid by Protein A chromatography. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide. Also available without BSA and azide at 1mg/ml.
Refrigerate at 4°C. Do not freeze.
Material Safety Data:
This product is not licensed or approved for administration to humans or to animals other than the experimental animals. Standard Laboratory Practices should be followed when handling this material. The chemical, physical, and toxicological properties of this material have not been thoroughly investigated. Appropriate measures should be taken to avoid skin and eye contact, inhalation, and ingestion. The material contains 0.09% sodium azide as a preservative. Although the quantity of azide is very small, appropriate care should be taken when handling this material as indicated above. The National Institute of Occupational Safety and Health has issued a bulletin citing the potential explosion hazard due to the reaction of sodium azide with copper, lead, brass, or solder in the plumbing systems. Sodium azide forms hydrazoic acid in acidic conditions and should be discarded in a large volume of running water to avoid deposits forming in metal drainage pipes.