ALK/p80- Rabbit Monoclonal

Recognizes a human p80 protein, identified as a hybrid of the anaplastic lymphoma kinase (ALK) gene and the nucleophosmin (NPM) gene resulting from the t(2;5)(p23;q35) translocation found in a third of large cell lymphomas. This rebbit polyclonal antibody can be used to detect p80 in these lymphomas and may also be used to detect a recently described subtype of large B cell lymphoma, which expresses the full-length ALK protein.

Immunohistochemistry (Formalin/paraffin) Working dilution: 1:100 for 10 min at RT. Staining of formalin-fixed tissues is required by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

The optimal dilution for a specific application should be determined by the investigator.

Recombinant protein corresponding to a region, which spans the tyrosine kinase catalytic domain and part of the C-terminus of the NPM-ALK transcript.

Cellular Localization:
Cytoplasmic and/or nuclear

Species Reactivity:
Human, others not tested.

Recommended Positive Control:
Anaplastic lymphoma.

Not determined

Tissue culture supernatant with 0.1% azide.

Aliquoting Instructions:
Dilute as recommended in application section with our primary antibody dilution buffer (M35) or any other buffer containing carrier protein.

Antibody Form:
Cell culture supernatant

This antibody reacts with Alk/p80 protein in IHC.