ALK/p80- Rabbit Monoclonal
Recognizes a human p80 protein, identified as a hybrid of the anaplastic lymphoma kinase (ALK) gene and the nucleophosmin (NPM) gene resulting from the t(2;5)(p23;q35) translocation found in a third of large cell lymphomas. This rebbit polyclonal antibody can be used to detect p80 in these lymphomas and may also be used to detect a recently described subtype of large B cell lymphoma, which expresses the full-length ALK protein.
Immunohistochemistry (Formalin/paraffin) Working dilution: 1:100 for 10 min at RT. Staining of formalin-fixed tissues is required by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
The optimal dilution for a specific application should be determined by the investigator.
Recombinant protein corresponding to a region, which spans the tyrosine kinase catalytic domain and part of the C-terminus of the NPM-ALK transcript.
Cytoplasmic and/or nuclear
Human, others not tested.
Recommended Positive Control:
Tissue culture supernatant with 0.1% azide.
Dilute as recommended in application section with our primary antibody dilution buffer (M35) or any other buffer containing carrier protein.
Cell culture supernatant
This antibody reacts with Alk/p80 protein in IHC.