Adenovirus Type 5 E1A
Reacts with 293 cells or adenovirus infected cells and tissues. The early region (E1A) of the adenovirus genome plays a central role in cellular transformation and regulation of gene expression. The E1A region encodes a series of related proteins (35-46 kD) with multifuctional capabilities and form a specific complex.
- Immunofluorescence (0.2-0.4µg/ml)
- Immunoprecipitation (Native verified)(Use Protein A; Ab at 2µg/mg protein lysate)
- Western Blotting (0.5-1.0µg/ml for 2 hrs at RT)
- Immunohistology (Use Ab at 2-4µg/ml for 30 min at RT)
- Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
-THE OPTIMAL DILUTION SHOULD BE DETERMINED THE INDIVIDUAL LAB
Adenovirus. Others not tested.
Recommended Positive Control:
293 Cells or Adenovirus infected cells and tissues
200ug/ml antibody purified from the ascites fluid by Protein A chromatography. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide. Also available without BSA and azide at 1mg/ml.
Refrigerate at 4°C. Do not freeze.
Material Safety Data:
This product is not licensed or approved for administration to humans or to animals other than the experimental animals. Standard Laboratory Practices should be followed when handling this material.
The chemical, physical, and toxicological properties of this material have not been thoroughly investigated. Appropriate measures should be taken to avoid skin and eye contact, inhalation, and ingestion. The material contains 0.09% sodium azide as a preservative.
Although the quantity of azide is very small, appropriate care should be taken when handling this material as indicated above. The National Institute of Occupational Safety and Health has issued a bulletin citing the potential explosion hazard due to the reaction of sodium azide with copper, lead, brass, or solder in the plumbing systems.
Sodium azide forms hydrazoic acid in acidic conditions and should be discarded in a large volume of running water to avoid deposits forming in metal drainage pipes.