BAG-1/RAP46/HAP1- Monocolnal

BAG-1 is excellent for biochemical applications. BAG-1 is a multifunctional protein that blocks apoptosis and interacts with several types of proteins, including Bcl-2 family proteins, the kinase Raf-1, certain tyrosine kinase growth factor receptors, and steroid hormone receptors.

- Immunoprecipitation (Denatured verified)(Use Protein G; Ab at 2µg/mg protein lysate)
- Western Blotting (Ab 1µg/ml for 2hrs at RT)

Full length recombinant Human BAG-1 protein

Cellular Localization:
p50 in Nucleus p36 in Cytoplasm

Species Reactivity:
Human. Others not tested.

Recommended Positive Control:
SKBR-3 Cells

Not determined

200ug/ml of antibody purified from ascites fluid by Protein G chromatography. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide. Also available without BSA and azide at 1mg/ml.

Refrigerate at 4°C. Do not freeze.

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2. Hohfeld J: Biol Chem 1998 Mar;379(3):269-274.
3. Takayama S, Xie Z, Reed JC: An Evolutionarily Conserved Family of Hsp70/Hsc70 Molecular Chaperone Regulators. J Biol Chem 1999 Jan 8;274(2):781-786.
4. Bimston D, Song J, Winchester D, Takayama S, Reed JC, Morimoto RI: BAG-1, a negative regulator of hsp70 chaperone activity, uncouples nucleotide hydrolysis from substrate release. EMBO J 1998 Dec 1;17(23):6871-6878.
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12. Gleichmann M, Beinroth S, Reed JC, Krajewski S, Schulz JB, Wullner U, Klockgether T, Weller M: Potassium deprivation-induced apoptosis of cerebellar granule neurons: cytochrome c release in the absence of altered expression of Bcl-2 family proteins. Cell Physiol Biochem 1998;8(4):194-201.
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