BAG-1/RAP46/HAP1- Monocolnal
Description:
BAG-1 is excellent for biochemical applications. BAG-1 is a multifunctional protein that blocks apoptosis and interacts with several types of proteins, including Bcl-2 family proteins, the kinase Raf-1, certain tyrosine kinase growth factor receptors, and steroid hormone receptors.
Application:
- Immunoprecipitation (Denatured verified)(Use Protein G; Ab at 2µg/mg protein lysate)
- Western Blotting (Ab 1µg/ml for 2hrs at RT)
-THE OPTIMAL DILUTION SHOULD BE DETERMINED THE INDIVIDUAL LAB.
Immunogen:
Full length recombinant Human BAG-1 protein
Cellular Localization:
p50 in Nucleus p36 in Cytoplasm
Species Reactivity:
Human. Others not tested.
Recommended Positive Control:
SKBR-3 Cells
Epitope:
Not determined
Presentation:
200ug/ml of antibody purified from ascites fluid by Protein G chromatography. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide. Also available without BSA and azide at 1mg/ml.
Storage:
Refrigerate at 4°C. Do not freeze.
References:
1. Takayama S, et al. EMBO J 1997;16(16):4887-4896.
2. Hohfeld J: Biol Chem 1998 Mar;379(3):269-274.
3. Takayama S, Xie Z, Reed JC: An Evolutionarily Conserved Family of Hsp70/Hsc70 Molecular Chaperone Regulators. J Biol Chem 1999 Jan 8;274(2):781-786.
4. Bimston D, Song J, Winchester D, Takayama S, Reed JC, Morimoto RI: BAG-1, a negative regulator of hsp70 chaperone activity, uncouples nucleotide hydrolysis from substrate release. EMBO J 1998 Dec 1;17(23):6871-6878.
5. Bardelli A, Longati P, Albero D, Goruppi S, Schneider C, Ponzetto C, Comoglio PM: HGF receptor associates w ith the anti-apoptotic protein BAG-1 and prevents cell death. EMBO J 1996 Nov 15;15(22):6205-6212.
6. Luders J, Demand J, Schonfelder S, Frien M, Zimmermann R, Hohfeld J: Cofactor-induced modulation of the functional specificity of the molecular chaperone Hsc70. Biol Chem 1998 Oct;379(10):1217-1226. 7. Yang X, Chernenko G, Hao Y, Ding Z, Pater MM, Pater A, Tang SC: Human BAG-1/RAP46 protein is generated as four isoforms by alternative translation initiation and overexpressed in cancer cells. Oncogene 1998 Aug 27;17(8):981-989.
8. Takayama S, Sato T, Krajewski S, Kochel K, Irie S, Millan JA, Reed JC: Cloning and functional analysis of BAG-1: a novel Bcl-2-binding protein with anti-cell death activity. Cell 1995 Jan 27;80(2):279-284.
9. Reed JC, Zha H, Aime-Sempe C, Takayama S, Wang HG: Structure-function analysis of Bcl-2 family proteins. Regulators of programmed cell death. Adv Exp Med Biol 1996;406:99-112.
10. Adachi M, Torigoe T, Takayama S, Imai K: BAG-1 and Bcl-2 in IL-2 signaling. Leuk Lymphoma 1998 Aug;30(5-6):483-491.
11. Stuart JK, Myszka DG, Joss L, Mitchell RS, McDonald SM, Xie Z, Takayama S, Reed JC, Ely KR: Characterization of interactions between the anti-apoptotic protein BAG-1 and Hsc70 molecular chaperones. J Biol Chem 1998;273(35):22506-22514.
12. Gleichmann M, Beinroth S, Reed JC, Krajewski S, Schulz JB, Wullner U, Klockgether T, Weller M: Potassium deprivation-induced apoptosis of cerebellar granule neurons: cytochrome c release in the absence of altered expression of Bcl-2 family proteins. Cell Physiol Biochem 1998;8(4):194-201.
13. Froesch BA, Takayama S, Reed JC: BAG-1L protein enhances androge n receptor function. J Biol Chem 1998 May 8;273(19):11660-11666.
14. Takayama S, Krajewski S, Krajewska M, Kitada S, Zapata JM, Kochel K, Knee D, Scudiero D, Tudor G, Miller GJ, Miyashita T, Yamada M, Reed JC: Expression and location of Hsp70/Hsc-binding anti-apoptotic protein BAG-1 and its variants in normal tissues and tumor cell lines. Cancer Res 1998 Jul 15;58(14):3116-3131.
15. Kitada S, Andersen J, Akar S, Zapata JM, Takayama S, Krajewski S, Wang HG, Zhang X, Bullrich F, Croce CM, Rai K, Hines J, Reed JC: Expression of apoptosis-regulating proteins in chronic lymphocytic leukemia: correlations with In vitro and In vivo chemoresponses. Blood 1998;91(9):3379-3389.