Biotin- PC- Goat
Phycoprobe Phycocyanin (PC)-Capryol-Biotin
3.0 M Ammonium Sulfate, 50 mM Sodium Phosphate, 0.05% Sodium Azide, pH 7.0.
Before withdrawing aliquots from this suspension be sure to mix vial contents well. To remove ammonium sulfate, transfer needed aliquot into a small conical centrifuge tube. Centrifuge for 4 minutes at maximum speed. Remove clear supernatant by aspiration with a Pasteur’s pipet or by blotting with the tip of a tissue paper. Resuspend colored precipitate in 100 mM sodium phosphate pH 7.0 containing 5 mg/mL of bovine serum albumin.
Refrigerate in dark at 4°C. Do not freeze.
PROTEIN CARRIER: bovine serum albumin.
This phycocyanin conjugate is supplied as a suspension in 3.0 M ammonium sulfate. This environment appears to be best both for the preservation of the biliprotein as well as the stability of the conjugate. The precipitation was carried out in the presence of bovine serum albumin.
Total activity of conjugate: >90%
The biliprotein used in making this conjugate had the following characteristics:
Molar Extinction Coefficient at 620 nm: 5.8 x 105
Emission Max. 636 nm (using excitation at 600 nm)
Some spectral changes occur following conjugation of biliproteins to other macromolecules as well in their further binding to some surfaces. In our experience, the main change consists of an emission shift to higher wavelengths; no alteration has been observed in the optimum excitation wavelength.