Adenovirus Fiber (Monomer and Trimer)
Description:
Adenovirus capsid proteins (62 kD and 180-200 kD) are synthesized in the cytoplasm and transported to the nucleus for assembly into the virus particles. The three major capsid proteins (hexon, penton base, and fiber) are synthesized late in infection. Reacts with Adenovirus infected cells and tissues.
Application:
- Inhibition of Ad2 attachment (Not suitable)(2)
- Immunofluorescence(1)
- Western Blotting(1)
-THE OPTIMAL DILUTION SHOULD BE DETERMINED BY THE INDIVIDUAL LAB.
Immunogen:
UV-irradiated Ad2 virus
Cellular Localization:
Nuclear
Species Reactivity:
Adenovirus
Recommended Positive Control:
Adenovirus infected cells and tissues
Epitope:
N-terminus, probably the first 17aa.
Presentation:
0.2 mg/ml antibody purified from the ascites fluid by Protein A chromatography. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide. Also available without BSA and azide at 1mg/ml.
Storage:
Refrigerate at 4°C. Do not freeze.
Notes:
This ab. recognizes both monomers and trimers, as judged by reactivity with both boiled (monomers (62kDa) only) and unboiled (both monomers and trimers (180-200kDa bands)) fiber on Western blots and by indirect immunofluorescence studies of cells infected with a temperature-sensitive Ad5 fiber mutant (H5ts142) which does not form trimers at the nonpermissive temp. at both 32 C and 39 C(1).
Material Safety Data:
This product is not licensed or approved for administration to humans or to animals other than the experimental animals. Standard Laboratory Practices should be followed when handling this material. The chemical, physical, and toxicological properties of this material have not been thoroughly investigated. Appropriate measures should be taken to avoid skin and eye contact, inhalation, and ingestion. The material contains 0.09% sodium azide as a preservative. Although the quantity of azide is very small, appropriate care should be taken when handling this material as indicated above. The National Institute of Occupational Safety and Health has issued a bulletin citing the potential explosion hazard due to the reaction of sodium azide with copper, lead, brass, or solder in the plumbing systems. Sodium azide forms hydrazoic acid in acidic conditions and should be discarded in a large volume of running water to avoid deposits forming in metal drainage pipes.