Redusol (A non-specific blocking reagent for immunocytochemical staining procedures.)
Tissue subjected to immunocytochemical techniques may require to be treated with Redusol in order to remove undesirable non-specific background. When used as instructed, Redusol will diminish most non-specific background.
Warning: Redusol contains strong irritants. Use protective gears. Avoid contact with skin and clothes.
Redusol is a nonspecific background blocking reagent for immunocytochemical staining procedures. Tissues subjected to immunocytochemical techniques may require treatment with Redusol in order to remove undesirable nonspecific background. When used as instructed, Redusol™ will diminish most nonspecific background staining.
Store at room temperature.
Treatment of Tissue Sections with Redusol: 1. Deparaffinize the tissue sections.
2. Bring sections to 100% alcohol.
3. Blot 100% alcohol and introduce Endo/Blocker (Cat. No. M69).
4. Incubate for 5 minutes at room temperature.
5. Blot Endo/Blocker and introduce Auto/Iodine solution.
6. Incubate for 1 minute at room temperature.
7. Introduce 1X Automation Buffer (Cat. No. M30). Incubate for 1 minute and blot.
8. Apply Redusol to tissue sections. Incubate for 5 minutes at 37°C or 45°C.
9. Wash specimens 5 times with 1X Automation Buffer.
10. Proceed with staining protocol.
MicroProbe Procedure: Place this Redusol reagent after the Auto/Iodine Reagent Bucket. Use protocol mentioned above. After Auto/Iodine treatment of the tissue sections, wash specimens twice with 1X Automation or Immunoassay Buffer. Next, treat the slides with Redusol and incubate for 5 minutes at 37°C or 45°C. Then, wash well with 1X Automation or 1X Immunoassay Buffer. Proceed with staining protocol.
Code-On Automated Procedure: Dedicate an Isolon strip to this reagent. Apply following the treatment with the Auto/Iodine solution. Introduce the above mentioned protocol into the particular software you are already using.