Blood Group Antigen B

Monoclonal Mouse Anti-Human Blood Group Antigen B

Immunohistochemistry 1:50-1:100.
The optimum conditions should be detdrmined by the individual lab.

Blood Group B erythrocytes.

Species Reactivity:
Human. Others not tested.

Recommended Positive Control:
Human Tissue (Blood Group B or AB)

20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, and 0.05% Sodium Azide, pH 7.5.

Aliquoting Instructions:
Do not dilute the entire reconsituted solution at once. Withdraw aliquots as needed with a micropipette and keep concentrated stock at 4°C. Dilute according to the particular application being used. In general, the 0.05M borate pH 8.0 containing 0.15M sodium chloride, 0.02% sodium azide, is a good dilutent to use with most antibodies. When diluting for immunohistochemistry, ELISA or western blot, make the dilution in Primary Antibody Diluting Buffer (Cat. No. M35). Avoid diluting the entire contents of the vial at once since the diluted solution may have reduced stability.

Staining Procedure:
This antibody can be used on frozen and formalin-fixed paraffin-embedded tissue sections. When using on formalin-fixed paraffin-embedded tissues a antigen demasking procedure is recommended.The antibody may be used at a dilution of 1:50-1:100 with AutoProbe III (Cat. No. 08-803). The optimal conditions should be determined by the individual laboratory.

This antibody stains erythrocytes and vascular epthelium of blood group B controls.

This antibody is compatible with the following Biømeda staining kits: AutoProbe III Wide Spectrum Universal Staining kits (Cat. No. 08-803, 08-804X), HistoScan Monoclonal Detector (Cat. No. 06-601, 06-601A and 06-601F). BiøStain Super ABC Mouse/Rat kits (Cat. No. 11-002 and 11-002A), UltraProbe High Sensitivity Staining kits (Cat. 09-900, 09-901, 09-901X), LAB/Probe Mouse/Rabbit kits (Cat. No. 10-101 and 10-102) and AutoProbe II Universal kits
(Cat. No. 08-801 and 08-802X).

Refrigerate at 4°C. Do not freeze.

1. Bundle, et al., J Immunol, 129: 678, 1982.
2. Chapman, et al., J Histochem Cytochem, 31: 557, 1983.
3. J. Va’ak, etal, Neoplasma 36:479-487, 1989.