B-cell (Pan-B)

Monoclonal Mouse Anti-Human B-cell (Pan B)

Myeloma X63 Ag 8.653.

20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, and 0.05% Sodium Azide, pH 7.5.

Aliquoting Instructions:
Do not dilute the entire reconsituted solution at once. Withdraw aliquots as needed with a micropipette and keep concentrated stock at 4°C. Dilute according to the particular application being used. In general, the 0.05M borate pH 8.0 containing 0.15M sodium chloride, 0.02% sodium azide, is a good dilutent to use with most antibodies. When diluting for immunohistochemistry, ELISA or western blot, make the dilution in Primary Antibody Diluting Buffer (Cat. No. M35). Avoid diluting the entire contents of the vial at once since the diluted solution may have reduced stability.

Staining Procedure:
This antibody can be used on formallin fixed, paraffin-embedded tissue sections, as well as on frozen tissue sections. For a three-step avidin-biotin complex detection system, a dilution of 1:10 is recommended as a guideline. When using paraffin-embedded and formalin-fixed tissues, high temperature antigenic unmasking technique (see instructions below) is strongly recommended for consistent and reproducible results. This product can also be used on frozen tissue sections. The optimal conditions should be determined by the individual laboratory.

This antibody is specific against a 45kD antigen that is located in the membranes of the human B-cells, a subset of T-cells, histiocytes and macrophages.

This antibody is compatible with the following Biømeda staining kits: AutoProbe III Wide Spectrum Universal Staining kits (Cat. No. 08-803, 08-804X), HistoScan Monoclonal Detector (Cat. No. 06-601, 06-601A and 06-601F.). BiøStain Super ABC Mouse/Rat kits (Cat. No. 11-002 and 11-002A), UltraProbe High Sensitivity Staining kits (Cat. 09-900, 09-901, 09-901X), LAB/Probe Mouse/Rabbit kits (Cat. No. 10-101 and 10-102) and AutoProbe II Universal kits (Cat. No. 08-801 and 08-802X).

Refrigerate at 4°C. Do not freeze.

1. Al Saati, T. et al. Blood, 1989, 74(7): 2476-2485.