Actin, Muscle - Prediluted

Recognizes a 42 kD protein alpha and gamma actin from skeletal, cardiac and smooth muscle cells but is non-reactive with beta isomers. This antibody also recognizes myocardial, skeletal and smooth muscle cells, as well as, myoepithelial cells, pericytes of small vessels. All other non-muscle cells are negative.

Immunohistochemistry (Paraffin/Frozen)

Actin isolated from Human Myocardium

Cellular Localization:

Recommended Positive Control:
Skeletal Cardiac and Smooth Muscle Granulocytes

Prediluted in Primary Antibody Diluting Buffer, pH 7.6.

Staining Procedure:
For paraffin-embedded tissue sections, we recommend an incubation time and temperature of 30 minutes at 37°C for this antibody, when used in conjunction with biømeda’s immunoperoxidase staining kit. *Prolonged fixation in buffered formalin can destroy the epitope.

This antibody is compatible with the following biømeda staining kits: AutoProbe III Wide spectrum Universal Staining Kits (Cat. No. 08-803, 08-804X), HistoScan Monoclonal Detector (Cat. No. 06-601, 06-601A and 06-601F), Biøstain Super ABC Mouse/Rat Kits (Cat. No. 11-002 and 11-002A), UltraProbe High Sensitivity Staining Kits (Cat. No. 09-900, 09-901, 09-901X).

Refrigerate at 4°C. Do not freeze.