Allophycocyanin (APC) unconjugated

Allophycocyanin (APC) unconjugated

50 mM Sodium Phosphate, 3.0 M Ammonium Sulfate, 0.05% Sodium Azide, pH 7.0.

Aliquoting Instructions:
Before withdrawing aliquots from this suspension be sure to mix vial contents well. To remove ammonium sulfate, transfer needed aliquot into a small conical centrifuge tube.

Centrifuge for 4 minutes at maximum speed. Remove clear supernatant by aspiration with a Pasteur’s pipet or by blotting with the tip of a tissue paper. Resuspend colored precipitate in 100 mM sodium phosphate pH 7.0 or in any other appropriate buffer. If complete removal of ammonium sulfate is required, dialyze solution against buffer or choice or desalt solution by molecular sieve chromatography.

Refrigerate in dark at 4°C. Do not freeze.

This phycobiliprotein is supplied as a suspension in 3.0 M ammonium sulfate. This environment appears to be best for the preservation of the biliprotein. The precipitation was carried out in the absence of any protein carrier.

The biliprotein in this product has the following characteristics:
Native allophcocyanin of type C.
Mol. Wt. 104,000
Molar Extinction Coefficient at 652 nm: 6.9 x 105
Emission Max. 660nm (using excitation at 652nm)
Absorbance ratio 650nm/280nm = 4.2