Activin Receptor, Type II (actRII)
Description:
The activins and inhibins are glycoproteins that belong to the transforming growth factor-b-like (TGF-b-like) superfamily of growth factors. These factors transduce their signals by interacting with two types of receptor serine/threonine kinases, that phosphorylate members of the SMAD family of transcription factors, thereby promoting their nuclear localization. Useful in the study of activin signal transduction pathways.
Application:
- Immunoprecipitation (Denatured verified)(Use Protein G; Ab 2µg/mg protein lysate)
- Western Blotting (Ab 1µg/ml for 2hrs at RT)
- THE OPTIMAL DILUTION SHOULD BE DETERMINED BY THE INDIVIDUAL LAB
Immunogen:
Recombinant human Type-2 Activin Receptor Tyrosine Kinase Domain
Cellular Localization:
Cell Membrane, Cytoplasmic
Species Reactivity:
Human. Others not tested.
Recommended Positive Control:
LS174T Cells
Epitope:
Tyrosine Kinase Domain
Presentation:
200ug/ml of antibody purified from ascites fluid by Protein G chromatography. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 15mM sodium azide. Also available without BSA and azide at 1mg/ml.
Storage:
Refrigerate at 4°C. Do not freeze.
Material Safety Data:
This product is not licensed or approved for administration to humans or to animals other than the experimental animals. Standard Laboratory Practices should be followed when handling this material.
The chemical, physical, and toxicological properties of this material have not been thoroughly investigated. Appropriate measures should be taken to avoid skin and eye contact, inhalation, and ingestion.
The material contains 0.09% sodium azide as a preservative. Although the quantity of azide is very small, appropriate care should be taken when handling this material as indicated above. The National Institute of Occupational Safety and Health has issued a bulletin citing the potential explosion hazard due to the reaction of sodium azide with copper, lead, brass, or solder in the plumbing systems. Sodium azide forms hydrazoic acid in acidic conditions and should be discarded in a large volume of running water to avoid deposits forming in metal drainage pipes.