Endo/Blocker
Description:
Endo/Blocker (Endogenous Enzyme Blocker)
Application:
This Endogenous Enzyme Blocker is useful to suppress endogenous phosphatase and peroxidase-like enzyme activities found in many tissue preparations destined for immunocytochemical procedures. Routine treatment of tissue specimens with Endo/Blocker aids in the elimination of undesirable background staining when using these immunoenzymatic procedures. The treatment of tissue sections with Endo/Blocker does not result in any noticeable decrease of the antigenic characteristics of the specimen.
Specificity:
This endogenous enzyme blocker is useful for suppressing endogenous phosphatase and peroxidase-like enzyme activities found in many tissue preparations destined for immunocytochemical procedures. Routine treatment of tissue specimens with Endo/Blocker aids in the elimination of undesirable background staining when using these immunoenzymatic procedures. The treatment of tissue sections with Endo/Blocker does not result in any noticeable decrease of the antigenic characteristics of the specimen.
Storage:
Refrigerate at 4°C. Do not freeze.
Notes:
Preparation of Working Reagent:
Add 1 mL of concentrated Endogenous Enzyme Blocker to 5 mL of Reagent Alcohol. Discard diluted solution if not used within seven days or if solution turns a straw yellow color.
Treatment of Tissue Sections with Endogenous Enzyme Blocker
Paraffin Embedded Tissue Sections: Following the removal of paraffin with xylene or Hemo-De, run the slides through two baths of absolute Reagent Alcohol. Treat the slides with the Endo/Blocker Working Reagent for 5 minutes at room temperature or, for optimal blocking, at 37°C. Continue with the partial hydration step by immersing the specimens in 95% ethanol. Hydrate the tissue sections in 1X Automation Buffer (Cat. No. M30). Proceed with the usual staining protocol.
Frozen Sections: Treat the slides with the Endo/Blocker working solution for 5 minutes at room temperature prior to the hydration of the tissue sections in 1X Automation Buffer (Cat. No. M30). Proceed with the usual staining protocol.
MicroProbe Staining Procedure: Prepare the Endogenous Blocker Working Reagent in one of the reagent buckets. Follow protocol outlined above for either paraffin embedded or frozen sections. Incubate with the Endo/Blocker for 5 minutes at room temperature or, for maximum blocking activity, incubate with the blocker for 5 minutes at 37°C.
Code-On Automated Immunocytochemistry: Use protocols available from Biømeda, free of charge, to registered Code-On users. Follow instructions included in these protocols.