Peroxidase~Streptavidin
Description:
Peroxidase~Streptavidin
Application:
IHC:1:200-1:500(using chromogem/substrate)
ELISA:1,000-1:10,000(chromogen/substrate)
WB:1:2,000-1:8,000(chromogen/substrate)
WB:1:10,000-1:40,000(ECL)
Presentation:
50 mM Sodium Phosphate, 0.9% Sodium Chloride, BSA 10 mg/ml, 0.05% Thimerosal, pH 7.2.
Aliquoting Instructions:
Do not dilute the entire reconsituted solution at once. Withdraw aliquots as needed with a micropipette and keep concentrated stock at 4°C. Dilute according to the particular application being used. In general, the 0.05M borate pH 8.0 containing 0.15M sodium chloride, 0.02% thimerosal, is a good dilutent to use with most antibodies. GENERAL INFO: When diluting for immunohistochemistry, ELISA or western blot, make the dilution in Primary Antibody Diluting Buffer (Cat. No. M35). Avoid diluting the entire contents of the vial at once since the diluted solution may have reduced stability.
Storage:
Refrigerate at 4°C. Do not freeze.
Notes:
CONJUGATE CHARACTERISTICS:
Total activity of cojugate against immobilized biotin
> 95%.