Neuron Specific Enolase (NSE) - ELISA
Description:
Neuron Specific Enolase (NSE)
Specificity:
SUMMARY AND EXPLANATION OF TEST
The glycolytic enzyme enolase (2-phospho-D-glycerate hydrolyase) exists as several dimeric isoenzymes (aa, ab, bb and gg) composed of three distinct subunits: a,b, and g.
Three isoenzymes are found in human brain: aa, ag, and gg. The ag and gg-enolase isoenzymes are also known as neuron-specific enolase (NSE) as these isoenzymes initially were detected in neurons and neuroendocrine cells. Lung cancer is one of the most spread cancer forms with incidences about 50-100 per 100,000 population.
Approximately 20% of the lung cancer is small cell lung cancer. NSE has been shown to be a valuable tumor marker of neuroendocrine origin, particularly in small cell lung cancer and in neuroblastoma. Patients with small cell lung cancer show various proportions of ag and gg isoenzymes. The determination of NSE should detect ag and gg isoforms with the same sensitivity.
Notes:
MATERIALS PROVIDED
Microwell Strips (96 wells): Streptavidin coated wells.8x12 strips
Enzyme Conjugate (11ml): Anti-NSE Antibodies conjugated to horseradish peroxidase. Biotinylated capture antibody solution (11mL)
Solution A (11ml): Tetramethylbenzidine solution
Reference standard set (0.75 mL/each) calibrated to 5, 25, 50, 100, and 200 ug/L store in the freezer in aliquot. The Enzyme is not stable.
Sample Diluents or zero standard (11 mL)
Well holder for securing individual well.