Monoclonal Mouse Anti-Human bcl-2 Oncoprotein
Synthetic peptide sequence comprising amino acid 51-54 of bcl-2 oncoprotein.
Recommended Positive Control:
20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, and 0.05% Sodium Azide, pH 7.5.
Do not dilute the entire reconsituted solution at once. Withdraw aliquots as needed with a micropipette and keep concentrated stock at 4°C. Dilute according to the particular application being used. In general, the 0.05M borate pH 8.0 containing 0.15M sodium chloride, 0.02% sodium azide, is a good dilutent to use with most antibodies. When diluting for immunohistochemistry, ELISA or western blot, make the dilution in Primary Antibody Diluting Buffer (Cat. No. M35). Avoid diluting the entire contents of the vial at once since the diluted solution may have reduced stability.
This antibody can be used on formalin-fixed and paraffin-embedded tissue sections. For a three-step avidin-biotin complex system, a dilution of 1:40 may be used as a guideline. Overnight incubation at 4°C gives better results. Optimal results are achieved when B5 or Bouin's instead of formalin is used as a fixative.It is recommended that this product be used in frozen tissue sections. The optimal conditions should be determined by the individual laboratory. For best staining results, acetone should be the choice of fixative.
This antibody reacts with the 25 kD bcl-2 protein, an integral membrane protein which lies within the cell rather than on the cell surface.
Refrigerate at 4°C. Do not freeze.
1. Tsujimoto Y, and Croce CM, Proc Natl Acad Sci (USA), 83: 5214-8, 1986.
2. Cleary MJ, et al., Cell, 47: 19-28, 1986.
3. Pezzella F, et al., Am J Pathol, 137: 225-32, 1990.
4. Hockenbery D, et al., Nature, 348: 334-6, 1990.