Reacts with all six known vertebrate isoforms of actin (MW~42 kD). It also reacts with two cytoplasmic actins (beta, gamma), which are highly homologous to one another, but which differ from the muscle actins at about 25 amino acid residues.
- Immunoprecipitation (Denatured verified)(Use Protein G) (Ab 2 micrograms/mg protein lysate)
- Western Blotting (0.5 micrograms/ml for 2hrs at RT)
-IHC (0.5-1 micrograms/ml 30 Mins at RT)
- Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
-THE OPTIMAL DILUTION SHOULD BE DETERMINED BY THE INDIVIDUAL LAB
Chicken Gizzard Actin
Human, Cow, Mouse, Dog, Pig, Rabbit, Rat, Chicken, Dictyostelium discoideum, and Physarum polycephalum. Others not tested.
Recommended Positive Control:
200ug/ml of antibody purified from ascites fluid by Protein G chromatography. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 15mM sodium azide. Also available without BSA and azide at 1mg/ml.
Refrigerate at 4°C. Do not freeze.
Material Safety Data:
This product is not licensed or approved for administration to humans or to animals other than the experimental animals. Standard Laboratory Practices should be followed when handling this material. The chemical, physical, and toxicological properties of this material have not been thoroughly investigated. Appropriate measures should be taken to avoid skin and eye contact, inhalation, and ingestion.
The material contains 0.09% sodium azide as a preservative. Although the quantity of azide is very small, appropriate care should be taken when handling this material as indicated above.
The National Institute of Occupational Safety and Health has issued a bulletin citing the potential explosion hazard due to the reaction of sodium azide with copper, lead, brass, or solder in the plumbing systems. Sodium azide forms hydrazoic acid in acidic conditions and should be discarded in a large volume of running water to avoid deposits forming in metal drainage pipes.
1. Lessard J. (1988) Two Monoclonal antibodies to actin: One muscle reactive and one generally reactive. Cell Motility and Cytoskeleton 10: 349- 362.