Donkey Serum 10 mls
Use as protein blocking agent in ELISA, WB and Immunohistochemistry-----2-10% in PBS
All sera are obtained from pools of healthy adult animals. These sera are delipidated, heat inactivated at 56C for 60 minutes, centifuged and filtered. Eanh serum is tested with appropriate antibody in ELISA system for possible cross-reactivity. It is supplied undiluted in 0.1% sodium azide as a preservative.
Refrigerate at 4°C.
For long term storage aliquot and store at -20C.
Avoid repeated freezing and thawing as it may precipitate proteins.
If precipitate is observed, withdraw aliquots as needed with pipet and transfer into conical microcentrifuge tube. Centifuge at max. speed for 2-3 mins. and use supernatant.