Bovine Serum Albumin -SPDP- Biotin conjugated
Description:
SPDP-(Biotin) Bovine Serum Albumin
Application:
CONJUGATING INSTRUCTIONS:
This new labeling system can be used in different ways but we recommend the following protocol:
The protein, peptide or polysaccharide to be labeled is first reacted with a thiolating reagent capable of introducing sulfhydro groups*, after rendered free of the unreacted reagents using dialysis or desalting techniques, it is mixed in predetermined proportions with the labeled BSA in same buffer as it is supplied in. The coupling reaction is carried for 25 hours at 4°C, after which, if desired, the unreacted materials can be separated from the newly-formed conjugate by molecular sieve chromatography. This conjugate is very stable providing that it is kept away from disulfide-reducing compounds. Add additional native BSA to act as a protein carrier and a preservative such as 0.02% sodium azide.
*Since an excess of sulfhydro groups incorporated on the material will have a tendency to produce pronounced crosslinking during the coupling step, it is recommended to first determine the optimal degree of substitution for each compound to be conjugated.
Presentation:
25 mM Sodium Borate, 150 mM Sodium Chloride, 0.05% Sodium Azide, pH 7.8.
Storage:
Refrigerate at 4°C. Do not freeze.
Notes:
CONJUGATE CHARACTERISTICS:
The bovine serum albumin (BSA) supplied as catalog number Y02 has been derivatized twice; once during the process of incorporating the N-succinimidyl 3-(2- pyridyldithio) propionate (SPDP) residues and again, during the labelling of the protein with Biotin. This SPDP-(Biotin)-BSA, due to its SPDP content, is reactive with sulfhydro groups.
Upon its binding to other proteins, peptides, hormones, polysaccharides etc., this special BSA will become a carrier for the biotin.This methodology eliminates the need for attaching the biotin derivatives directly on the compound being labeled, minimizing undesirable interactions of the material with biotin.CONJUGATING INSTRUCTIONS:
This new labeling system can be used in different ways but we recommend the following protocol:
The protein, peptide or polysaccharide to be labeled is first reacted with a thiolating reagent capable of introducing sulfhydro groups*, after rendered free of the unreacted reagents using dialysis or desalting techniques, it is mixed in predetermined proportions with the labeled BSA in same buffer as it is supplied in. The coupling reaction is carried for 25 hours at 4°C, after which, if desired, the unreacted materials can be separated from the newly-formed conjugate by molecular sieve chromatography.
This conjugate is very stable providing that it is kept away from disulfide-reducing compounds. Add additional native BSA to act as a protein carrier and a preservative such as 0.02% sodium azide.*Since an excess of sulfhydro groups incorporated on the material will have a tendency to produce pronounced crosslinking during the coupling step, it is recommended to first determine the optimal degree of substitution for each compound to be conjugated.KEEP UNUSED STOCK SOLUTION IN SUPPLIED VIAL AT 4°C .FOR RESEARCH USE ONLY. NOT INTENDED FOR USE IN HUMANS OR CLINICAL DIAGNOSIS.