Anti-Rat IgG (Mouse Ads.)- Rhodamine
Rhodamine-Anti-Rat IgG (Mouse Ads.)
50 mM Sodium Bicarbonate-Borate, 0.9% Sodium Chloride pH 8.1, 0.05% sodium azide.
Do not dilute the entire reconsituted solution at once. Withdraw aliquots as needed with a micropipette and keep concentrated stock at 4°C. Dilute according to the particular application being used. In general, the 0.05M borate pH 8.0 containing 0.15M sodium chloride, 0.02% sodium azide, is a good dilutent to use with most antibodies. When diluting for immunohistochemistry, ELISA or western blot, make the dilution in Primary Antibody Diluting Buffer (Cat. No. M35). Avoid diluting the entire contents of the vial at once since the diluted solution may have reduced stability.
Refrigerate in dark at 4°C. Do not freeze.
CROSS-REACTIVITY CHARACTERISTICS:NON-REACTIVE AGAINST MOUSE IgG.BUFFER COMPOSITION: 50 mM Bicarbonate-Borate Buffer 0.9% sodium chloride pH 8.1, 0.05% sodium azide.PROTEIN CARRIER: bovine serum albumin.CONJUGATE CHARACTERISTICS:The anti-rat IgG antibody used in this conjugation is an affinity purified antibody whichi was further treated with mouse IgG to remove cross-reacting antibodies. The binding acitivity of conjugate towards rat IgG is >95%.Degree of labeling:Ratio of OD 555/OD 280 = 0.58.lution can be diluted 20-100 times depending on the particular system employed. A recommended diluent is 5% solution of Normal Goat serum or 1% solution of bovine serum albumin in 50 mM phosphate-buffered saline pH 7.4, 0.05% sodium azide.Avoid storage of diluted solutions as they may decay with time.FOR CELL SORTING APPLICATIONS:Use 2-5 Micrograms of conjugate per 1 x 106 cells suspended in 0.1 mL.FOR RESEARCH USE ONLY. DO NOT USE IN DIAGNOSTIC PRODEDURES.