Pisum Sativum Agglutinin (PSA)

Description:
Pisum Sativum Agglutinin (PSA)

Immunogen:
Isolated from garden pea seeds.

Application:
PSA has been usd to fractionate cells, to isolate glycoproteins and glycopeptides, to distinguish between normal and virally transformed cells, as a T-cell mitogen, and as a an inhibitor of allograft rejection.

Presentation:
25 mM sodium borate-bicarbonate, 0.5 M Sodium Chloride 0.05% Sodium Azide, pH 7.8.

Specificity:
MAIN CARBOHYDRATE RECOGNITION CHARACTERISTICS: The lectin has specificity towards a-linked-mannose-containing-oligosaccharides, with an N-acetylchitobiose-linked alpha-fucose residue included in the receptor sequence.


Storage:
Refrigerate at 4°C. Do not freeze.

Notes:
The lectin used in this product is a research grade affinity-purified PSA, from garden pea seeds.
TOTAL ACTIVITY OF LECTIN AGAINST AN AFFINITY MATRIX: >90%.
MOLECULAR WEIGHT OF SUBUNITS RESOLVED BY SDS-PAGE:
49,000 d (2 subunits of 17,000 and 6,000 daltons each). PSA is nearly identical in structure and carbohydrate specificity to Lens culinaris agglutinin.
ALIQUOTING INSTRUCTIONS:
This solution contains 2.0 mg of lectin per milliliter. Dilute 100 to 500 fold in 50 mM sodium acetate buffer pH 6.5, 0.9% sodium chloride containing 1 mM CaCl2 and MnCl2( REQUIRED FOR ACTIVITY0). Use 2 mg/mL bovine serum albumin as a protein carrier. Do not dilute entire sample at once.