Actin, Alpha-Smooth Muscle - Prediluted
Description:
Monoclonal Mouse Anti-Human Alpha-Smooth Muscle Actin
Application:
This antibody is prediluted for Immunohistochemistry use.
Immunogen:
The N-terminal decapeptide of alpha-smooth muscle actin.
Cellular Localization:
cytoplasmic
Species Reactivity:
Human, Baboon, Rabbits. Others not tested.
Recommended Positive Control:
Bowel
Presentation:
Mouse monoclonal antibody prediluted in Primary Antibody Diluting Buffer pH 7.6.
Aliquoting Instructions:
Not applicable
Staining Procedure:
The prediluted antibody can be used on frozen cryostat sections as well as formalin-fixed paraffin-embedded tissue sections. For paraffin-embedded tissue sections, we recommend an incubation time and temperature of 30 minutes at 37°C for this antibody, when used in conjunction with Biømeda’s immunoperoxidase staining kit. *Prolonged fixation in buffered formalin can destroy the epitope.
Antibody Form:
Prediluted for IHC
Specificity:
This antibody reacts with the alpha-smooth muscle isoform of actin. It does not react with actin from fibroblasts (beta- and gamma-cytoplasmic), striated muscle (alpha-sarcomeric) and myocardium (alpha-myocardial).
Compatibility:
This antibody is compatible with the following Biømeda staining kits: AutoProbe III Wide Spectrum Universal Staining kits (Cat. No. 08-803, 08-804X), HistoScan Monoclonal Detector (Cat. No. 06-601, 06-601A and 06-601F.). BiøStain Super ABC Mouse/Rat kits (Cat. No. 11-002 and 11-002A), UltraProbe High Sensitivity Staining kits (Cat. 09-900, 09-901, 09-901X), LAB/Probe Mouse/Rabbit kits (Cat. No. 10-101 and 10-102) and AutoProbe II Universal kits (Cat. No. 08-801 and 08-802X).
Storage:
Refrigerate at 4°C. Do not freeze.
References:
1.Skalli O, Ropraz P, Trzeciak A, Benzonana G, Gillesen D, Gabbiani G. A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation. J Cell Biol 103: 2787-96, 1986.
2.Skalli O, Schürch W, Seemayer T, et al. Myofibroblasts from diverse pathologic settings are heterogenous in their content of actin isoforms and intermediate filament proteins. Lab Invest 60: 275-85,1989.
3. Skalli O, Pelte M-F, Peclet M-C, et al. Alpha-smooth muscle actin, a differentiation marker of smooth muscle cells, is present in microfilamentous bundles of pericytes. J Histochem Cytochem 37: 315-21, 1989.
4. Rannov-Jessen L, Van Deurs B, Celis JE, Petersen OW. Smooth muscle differentiation in cultured human breast gland stromal cells. Lab Invest 63: 532-43, 1990.
5. Hasegawa T, Hirose T, Kudo E, Abe J, Hizawa K. Cytoskeletal characteristics of myofibroblasts in benign and reactive fibroblastic lesions. Virochows Archiv (A) Pathol Anat 416:375-82, 1990.
6. Schmitt-Gräff A, Krüger S, Bochard F, Gabbiani G, Denk H. Modulation of alpha smooth muscle actin and desmin expression in perisinusoidal cells of normal and diseased human livers. Am J Pathol 138:1233-42, 1991.