Blood Group Antigen A - Concentrated
Monoclonal Mouse Anti-Human Blood Group Antigen A
Synthetic trisaccharide, alpha-D-N-Acetyl-galactosamine-(1-3)-(alpha-L-Fuc(1-2))-beta-D-Gal.
Human. Others not tested.
Recommended Positive Control:
20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, and 0.05% Sodium Azide, pH 7.5.
Do not dilute the entire reconsituted solution at once. Withdraw aliquots as needed with a micropipette and keep concentrated stock at 4°C. Dilute according to the particular application being used. In general, the 0.05M borate pH 8.0 containing 0.15M sodium chloride, 0.02% sodium azide, is a good dilutent to use with most antibodies. When diluting for immunohistochemistry, ELISA or western blot, make the dilution in Primary Antibody Diluting Buffer (Cat. No. M35). Avoid diluting the entire contents of the vial at once since the diluted solution may have reduced stability.
This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50 with AutoProbe III (Cat. No. 08-803). It is recommended that this product be used on frozen tissue sections or specimens. The optimal conditions should be determined by the individual laboratory.
This antibody reacts with blood group substance A, subtype A1 and A2, on the cell membranes of epithelium, endothelium and red blood cells.
This antibody is compatible with the following Biømeda staining kits: AutoProbe III Wide Spectrum Universal Staining kits (Cat. No. 08-803, 08-804X), HistoScan Monoclonal Detector (Cat. No. 06-601, 06-601A and 06-601F.). BiøStain Super ABC Mouse/Rat kits (Cat. No. 11-002 and 11-002A), UltraProbe High Sensitivity Staining kits (Cat. 09-900, 09-901, 09-901X), LAB/Probe Mouse/Rabbit kits (Cat. No. 10-101 and 10-102) and AutoProbe II Universal kits (Cat. No. 08-801 and 08-802X).
Refrigerate at 4°C. Do not freeze.
1. Chapman et al, J Histochem Cytochem, 31: 557, 1983.