Androgen Receptor- Mouse
The expression of AR, a 100 kD nuclear protein, is reportedly inversely correlated with histologic grade (i.e. well differentiated prostate tumors show higher expression than the poorly differentiated tumors). In prostate cancer, AR has been proposed as a marker of hormone-responsive.
- Gel Supershift (Order Ab at 1mg/ml)
- Immunoprecipitation (Denatured verified)(Use Protein G; Ab 2µg/mg protein lysate)
- Western Blotting (Ab 1µg/ml for 2hrs at RT)
- Immunohistology (Formalin/paraffin)(Ab 2-4µg/ml for 30 min at RT)
- Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0.
- THE OPTIMAL DILUTION SHOULD BE DETERMINED THE INDIVIDUAL LAB
A synthetic peptide from human AR
Human and Dog. Does not react with Mouse. Others not tested.
Recommended Positive Control:
LnCap Cells or Prostate Carcinoma
200ug/ml of antibody purified from ascites fluid by Protein G chromatography. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.
Refrigerate at 4°C. Do not freeze.
Material Safety Data:
This product is not licensed or approved for administration to humans or to animals other than the experimental animals. Standard Laboratory Practices should be followed when handling this material. The chemical, physical, and toxicological properties of this material have not been thoroughly investigated. Appropriate measures should be taken to avoid skin and eye contact, inhalation, and ingestion. The material contains 0.09% sodium azide as a preservative.
Although the quantity of azide is very small, appropriate care should be taken when handling this material as indicated above. The National Institute of Occupational Safety and Health has issued a bulletin citing the potential explosion hazard due to the reaction of sodium azide with copper, lead, brass, or solder in the plumbing systems.
Sodium azide forms hydrazoic acid in acidic conditions and should be discarded in a large volume of running water to avoid deposits forming in metal drainage pipes.
1. Ruizeveld de Winter J A, et al. (1991) J Histochem Cytochem 39: 927-936.
2. Chodak G w, et al. (1992) J Urol 147: 798-803.