Steptavidin, unconjugated

Description:
Streptavidin, unconj.

Application:
For conjugation with Peroxixidase, Alkaline phosphatase, Cy2, Cy3, Cy5, FITC, Rhodamine, Texas red, Biotin, R-PE, APC, etc.
ELISA (solid phase) apply 50 microliters at conc. of 2-5 micrograms/ml in HCO3 buffer overnight at 4C, followed by washing plate with PBS.
The optimum dilution should be determined by the individual lab.


Presentation:
10 mgs of streptavidin in solid form.

Form Supplied:
solid

Storage:
-20C

Notes:
Activity (biotin binding):>14.6 units/mg solid=15.3 units/mg solid
Reference Value:16.28 units/mg
Unit Defination: One unit of streptavidin will bind 1 microgram of biotin.
Protein content: 98 (+/- 5) %=98.08
HPLC Purity:>/= 99.7
Isoelectric point: approx. 7.0 determined by isoelectric focusing.
Protease activity: 0.000077 units/mg.