For conjugation with Peroxixidase, Alkaline phosphatase, Cy2, Cy3, Cy5, FITC, Rhodamine, Texas red, Biotin, R-PE, APC, etc.
ELISA (solid phase) apply 50 microliters at conc. of 2-5 micrograms/ml in HCO3 buffer overnight at 4C, followed by washing plate with PBS.
The optimum dilution should be determined by the individual lab.
10 mgs of streptavidin in solid form.
Activity (biotin binding):>14.6 units/mg solid=15.3 units/mg solid
Reference Value:16.28 units/mg
Unit Defination: One unit of streptavidin will bind 1 microgram of biotin.
Protein content: 98 (+/- 5) %=98.08
HPLC Purity:>/= 99.7
Isoelectric point: approx. 7.0 determined by isoelectric focusing.
Protease activity: 0.000077 units/mg.