Microphthalmia (Transcription Factor) (MiTF)
Mi is a basic helix-loop-helix-leucin zipper (b-HLH-ZIP) transcription factor implicated in pigmentation, mast cells, and bone development. The mutation of Mi causes Waardenburg Syndrome type II in humans.
- Gel Supershift (Order Ab at 1mg/ml)
- Western Blotting - Not verified
- Immunohistology (Formalin/paraffin)(Use Ab at 4-8 µg/ml for 30 min at RT)
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
-THE OPTIMAL DILUTION SHOULD BE DETERMINED BY THE INDIVIDUAL LAB.
N-terminal fragment of human Mi protein
Human, Dog. Does not react with Mouse, and Rat. Others not tested.
Recommended Positive Control:
501 Mel Cells (1), Melanoma
200ug/ml of antibody purified from ascites fluid by Protein G chromatography. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide. Also available without BSA and azide at 1mg/ml.
Refrigerate at 4°C. Do not freeze.
This ab. does not cross-react with other b-HLH-ZIP factors by DNA mobility shift assay. This ab. reacts with both melanocytic and nonmelanocytic isoforms of Mi. It is especially useful for research on murine Mi protein.
1. Weilbaecher KN, et al. J. Exp.Med. 1998, 187: 775-785
2. Hemesath P, et. al. Nature. 1998, 391:298-301