Bcl-2, Bax, and Bcl-X are members of a family of proteins that regulate apoptosis. In contrast to Bcl-2 and Bcl-XL, overexpression of bax protein accelerates the programmed cell death (apoptosis).
- Immunoprecipitation (Not suitable)
- Western Blotting (Ab 1µg/ml for 2hrs at RT)
-THE OPTIMAL DILUTION SHOULD BE DETERMINED BY THE INDIVIDUAL LAB
A Synthetic peptide, aa 3-16 (Cys-GSGEQLGSGGPTSS), of Mouse bax protein. This amino acid sequence is NOT shared by rat and human bax protein.
Cytoplasmic and Cell Membrane
Mouse. Does not react with Rat and Human. Others not tested.
Recommended Positive Control:
MAD109 or Schmidt-Rubin Cells
200ug/ml antibody purified from the ascites fluid by Protein G chromatography. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide. Also available without BSA and azide at 1mg/ml.
Refrigerate at 4°C. Do not freeze.
Material Safety Data:
This product is not licensed or approved for administration to humans or to animals other than the experimental animals. Standard Laboratory Practices should be followed when handling this material. The chemical, physical, and toxicological properties of this material have not been thoroughly investigated. Appropriate measures should be taken to avoid skin and eye contact, inhalation, and ingestion. The material contains 0.09% sodium azide as a preservative. Although the quantity of azide is very small, appropriate care should be taken when handling this material as indicated above. The National Institute of Occupational Safety and Health has issued a bulletin citing the potential explosion hazard due to the reaction of sodium azide with copper, lead, brass, or solder in the plumbing systems. Sodium azide forms hydrazoic acid in acidic conditions and should be discarded in a large volume of running water to avoid deposits forming in metal drainage pipes.
1. Hsu YT, et. al. J Bio Chem, 1997, 272(21):13829-34.
2. Hsu YT, et. al. Proc Natl Acad Sci USA, 1997, 94(8):3668-72.