Actin, Alpha-Smooth Muscle - Dehydrated
Monoclonal Mouse Anti-Human Alpha-Smooth Muscle Actin
The N-terminal decapeptide of alpha-smooth muscle actin.
Human, Baboon, Rabbits. Others not tested.
Recommended Positive Control:
The Biømeda K002 is supplied in two screw cap microtubes, labeled A and B. Tube A, which has an orange cap, contains 0.5 mL of dehydrated antibody reconstitution buffer. Tube B, which has a blue cap, contains the dehydrated concentrated monoclonal antibody. The light green color is the combination of No. 1 blue and No. 5 yellow food dyes, it serves as an indicator for the reconstitution procedure.
Do not dilute the entire reconsituted solution at once. Withdraw aliquots as needed with a micropipette and keep concentrated stock at 4°C. Dilute according to the particular application being used. In general, the 0.05M borate pH 8.0 containing 0.15M sodium chloride, 0.02% sodium azide, is a good dilutent to use with most antibodies. GENERAL INFO: When diluting for immunohistochemistry, ELISA or western blot, make the dilution in Primary Antibody Diluting Buffer (Cat. No. M35). Avoid diluting the entire contents of the vial at once since the diluted solution may have reduced stability.
This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:20 with AutoProbe III (Cat. No. 08-803). It is recommended that this product be used on frozen tissue sections or specimens. The optimal conditions should be determined by the individual laboratory.
This antibody reacts with the alpha-smooth muscle isoform of actin. It does not react with actin from fibroblasts (beta- and gamma-cytoplasmic), striated muscle (alpha-sarcomeric) and myocardium (alpha-myocardial).
This antibody is compatible with the following Biømeda staining kits: AutoProbe III Wide Spectrum Universal Staining kits (Cat. No. 08-803, 08-804X), HistoScan Monoclonal Detector (Cat. No. 06-601, 06-601A and 06-601F.). BiøStain Super ABC Mouse/Rat kits (Cat. No. 11-002 and 11-002A), UltraProbe High Sensitivity Staining kits (Cat. 09-900, 09-901, 09-901X), LAB/Probe Mouse/Rabbit kits (Cat. No. 10-101 and 10-102) andAutoProbe II Universal kits (Cat. No. 08-801 and 08-802X).
Refrigerate at 4°C. Do not freeze.
1. Skalli O, Ropraz P, Trzeciak A, Benzonana G, Gillesen D, Gabbiani G. A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation. J Cell Biol 103: 2787-96, 1986.
2. Skalli O, Schürch W, Seemayer T, et al. Myofibroblasts from diverse pathologic settings are heterogenous in their content of actin isoforms and intermediate filament proteins. Lab Invest 37: 315-21,1989.
3. Skalli O, Pelte M-F, Peclet M-C, et al. Alpha-smooth muscle actin, a differentiation marker of smooth muscle cells, is present in microfilamentous bundles of pericytes. J Histochem Cytochem 37: 315-21, 1989