Tetramethylbenzidine (TMB) - Ready to Use Chromogen - 5x100 ml
Tetramethylbenzidine (TMB) Ready-to Use Chromogen (Ready-to-use Peroxidase Chromogen Substrate for ELISA )
3-3’,5,5'-tetramethylbenzidine (TMB) is a soluble chromogen substrate for horseradish peroxidase detection systems. It is recommended for ELISA procedures. Biømeda Ready-to-Use TMB is a combination of buffer, chromogen and H2O2, as a substrate, in one convenient single component reagent. The use of TMB as a chromogen, when peroxidase-based immunochemical procedures are employed, provides the means of visualisation and measurement of analytical concentration or enzyme activity in quantitative assays.
TMB yields soluble blue end product that can be analyzed kinetically using the microtiter plate reader at wavelength of 650 nm. Reaction can be stopped by addition of 0.5 M sulfuric acid or 1.0 M Hydrochloric acid yielding in deep yellow color and read at 450 nm. When evaluating end point reaction visually, consider deep yellow color as positive and clear as negative.
TMB substrate is much more sensitive and less hazardous than benzidine or its other derivatives traditionally used as HRP chromogen substrates.
TMB is a ready to use chromogen for ELISA only. It is not suitable for immunohistochemistry.
Refrigerate at 4°C. Do not freeze.