Amyloid A Component - Concentrated
Description:
This antibody reacts with native and fixed amyloid fibrils in paraffin-embedded tissue sections. This antibody recognizes amyloid deposits in all human organs and tissues including kidney and rectum.
Application:
Immunohistochemistry (Paraffin/Frozen)
Immunogen:
Human Amyloid Protein A
Cellular Localization:
Extracellular
Species Reactivity:
Human. Others not tested.
Presentation:
20 mM Tris-Borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing Fetal Bovine Serum, BMC-6 Carrier Polysaccharides, Carrier Protein, and 0.05% Sodium Azide, pH 7.5.
Aliquoting Instructions:
Do not dilute the entire reconsituted solution at once. Withdraw aliquots as needed with a micropipette and keep concentrated stock at 4°C. Dilute according to the particular application being used. In general, the 0.05M Borate pH 8.0 containing 0.15M Sodium Chloride, 0.05% Sodium Azide, is a good dilutent to use with most antibodies. When diluting for Immunohistochemistry, ELISA, or Western Blot, make the dilution in Primary Antibody Diluting Buffer (Cat. No. M35). Avoid diluting the entire contents of the vial at once since the diluted solution may have reduced stability.
Staining Procedure:
Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:25 with AutoProbe III (Cat. No. 08-803). For a three-step avidin-biotin complex system with extensive incubation protocols, a dilution of 1:50 to 1:100 may be used as a guideline. The optimal conditions should be determined by the individual laboratory.
Compatibility:
This antibody is compatible with the following biømeda staining kits: AutoProbe III Wide Spectrum Universal Staining kits (Cat. No. 08-803, 08-804X), HistoScan Monoclonal Detector (Cat. No. 06-601, 06-601A and 06-601F.). BiøStain Super ABC Mouse/Rat kits (Cat. No. 11-002 and 11-002A), UltraProbe High Sensitivity Staining kits (Cat. 09-900, 09-901, 09-901X), Lab/Probe Mouse/Rabbit kits (Cat. No. 10-101 and 10-102) and AutoProbe II Universal kits (Cat. No. 08-801 and 08-802X).
Storage:
Refrigerate at 4°C. Do not freeze.
References:
1. Linke R.P. Blut 45: 407-409, 1982.
2. Linke R.P. J Histochem Cytochem 32: 322-8, 1984.